Journal: bioRxiv

Article Title: β-Cyclodextrins as affordable antivirals to treat coronavirus infection

doi: 10.1101/2022.11.16.516726

Figure Lengend Snippet: MRC-5 cells were absorbed with HCoV-229E at MOI of 0.1 PFUs/cell for 1h, exposed to increasing concentrations of the drug for 24h and processed by immunofluorescence with antibodies specific for the HCoV-229E N protein and with an anti-rabbit secondary antibody conjugated with Alexa fluor 488 (green). Nuclei were labeled with DAPI (blue). Images were collected with an epifluorescence microscope. ( A ) Representative pictures of the immunofluorescence assay. Scale bars, 100 μm. ( B ) Dose-response curves (red lines) of OSW-1, HβCD, U18666A, Phytol, Mdivi-1, FLI 06, Baclogen and CI976 were determined by nonlinear regression. Data is shown as mean ± S.E.M. of 3 biological replicates. Cytotoxic effect on MRC-5 cells exposed to increasing concentrations of drugs in the absence of virus is also shown (black lines).

Article Snippet: Images were obtained with a Leica DMi8 S widefield epifluorescence microscope and processed with Image J software.

Techniques: Immunofluorescence, Labeling, Microscopy, Virus

Journal: bioRxiv

Article Title: Chronic alteration of Ca 2+ and hemodynamic signals induced by intracortical microstimulation in the visual cortex of awake mice

doi: 10.1101/2025.10.23.679242

Figure Lengend Snippet: ( A ) Timeline of chronic recordings. ( B ) Imaging and stimulation setups. Widefield and two-photon imaging was performed on the implanted hemisphere, and visual stimulation was applied to the contralateral eye. ( C ) Example widefield images of neural (Ca 2+ dF/F 0 ) and OEF hemodynamic responses induced by 25-Hz ICMS. Scale bar = 500 µm. ( D and E ) Time course (left) and distance profile (right; measured at 5 [red] and 20 [blue] seconds after stimulation onset) of induced neural (D) and hemodynamic (E) responses. Mean ± SD across four trials. Shaded bar indicates stimulation period (0-10 s); vertical dashed lines mark the time points of distance profiles.

Article Snippet: We employed a mesoscopic-scale widefield microscope (MVX-10 epifluorescence microscope, Olympus, Tokyo Japan) for simultaneous recording of Ca 2+ fluorescent and intrinsic optical signals , , .

Techniques: Imaging

Journal: bioRxiv

Article Title: Chronic alteration of Ca 2+ and hemodynamic signals induced by intracortical microstimulation in the visual cortex of awake mice

doi: 10.1101/2025.10.23.679242

Figure Lengend Snippet: ( A ) Coefficient of variation (CV, pixel-wise standard deviation normalized by mean over time) of widefield Ca 2+ (i) and hemodynamics (ii) during pre-stimulation periods at day 0 (top) and day 14 (bottom). ( B ) CV (top) and silencing index relative to 350 µm (SI 350 = [CV d -CV 350 ]/CV 350 , where d = 50, 150, 250, 350, or 450 μm) during pre-stimulation periods (bottom) across distances and imaging days. ( C ) CV map during visual stimulation. ( D ) CV and SI 350 during visual stimulation. * in SI 350 plots indicate significant differences from 0 (one-sample t-test with Bonferroni correction, p < 0.05; colors correspond to days after implantation).

Article Snippet: We employed a mesoscopic-scale widefield microscope (MVX-10 epifluorescence microscope, Olympus, Tokyo Japan) for simultaneous recording of Ca 2+ fluorescent and intrinsic optical signals , , .

Techniques: Standard Deviation, Imaging